Document 0157
 DOCN  M9490157
 TI    Evaluation of monoclonal antibodies to HIV-1 envelope by neutralization
       and binding assays: an international collaboration.
 DT    9411
 AU    D'Souza MP; Geyer SJ; Hanson CV; Hendry RM; Milman G; Division of AIDS,
       National Institute of Allergy and Infectious; Diseases, National
       Institutes of Health, Bethesda, MD 20892.
 SO    AIDS. 1994 Feb;8(2):169-81. Unique Identifier : AIDSLINE MED/94318200
 AB    OBJECTIVE: To characterize a purified panel of monoclonal antibodies
       (MAb) to epitopes in HIV-1 envelope V3, CD4-binding region, C4 and gp41.
       DESIGN: Neutralization and/or binding activity data were obtained from
       21 laboratories on a coded panel consisting of seven human MAb, seven
       mouse MAb, recombinant human CD4 immunoadhesin [CD4-immunoglobulin G
       (IgG)], normal human and normal murine Ig. METHODS: Laboratories
       performed a variety of neutralization assays and antigen binding assays
       with HIVIIIB, HIVMN and other laboratory strains of HIV-1. RESULTS: For
       a single MAb, there was up to a 10(3) range of neutralizing antibody
       titers between laboratories. The range in titers appeared to depend on
       the sensitivity of the neutralization assay. Two methods were used to
       consolidate the data from all laboratories, the geometric mean titer
       (GMT) and the median neutralizing titer (MNT). The panel of MAb were
       also analyzed by a variety of assays that measure binding activity to
       native or denatured epitopes. The relative binding activity of the MAb
       did not appear to correlate with neutralizing activity. CONCLUSION:
       Neutralization results from any single laboratory did not correlate with
       the collective data. The relative potency (rank order) of the MAb in the
       panel were equivalent when determined by GMT or MNT. These values may be
       useful to individual laboratories for estimating the sensitivity of
       their neutralization assays. The study also identified potential
       reference reagents with which neutralizing activity could be compared.
 DE    Amino Acid Sequence  Animal  Antibodies, Monoclonal/*IMMUNOLOGY
       Antigen-Antibody Reactions  Antigenic
       Determinants/CHEMISTRY/IMMUNOLOGY/METABOLISM  Antigens, CD4/METABOLISM
       Binding Sites  Comparative Study  CHO Cells  Enzyme-Linked Immunosorbent
       Assay  Gene Products, env/IMMUNOLOGY/METABOLISM  Hamsters  Human  HIV
       Antibodies/*IMMUNOLOGY/METABOLISM  HIV Antigens/*IMMUNOLOGY/METABOLISM
       HIV Envelope Protein gp120/IMMUNOLOGY/METABOLISM  HIV Envelope Protein
       gp41/IMMUNOLOGY/METABOLISM  HIV-1/*IMMUNOLOGY  International Cooperation
       Mice  Molecular Sequence Data  Neutralization Tests  Peptide
       Fragments/IMMUNOLOGY/METABOLISM  Protein Binding  Protein
       Precursors/IMMUNOLOGY/METABOLISM  Recombinant
       Proteins/IMMUNOLOGY/METABOLISM  Reference Standards  Reproducibility of
       Results  Saccharomyces cerevisiae  Sensitivity and Specificity  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE
       MULTICENTER STUDY

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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